Subcellular localization and in vivo subunit interactions of ubiquitous -calpain
نویسندگان
چکیده
1 Abteilung für Klinische Chemie und Klinische Biochemie, Chirurgische Klinik Innenstadt, Klinikum der LMU München, D-80336 München 2 Adolf-Butenandt-Institut der LMU München, D-80336 München 3 Deutsches Krebsforschungszentrum, D-69120 Heidelberg 4 Physiologisches Institut der LMU München, D-80336 München 5 Institut für Molekularbiologie und Zellkulturtechnik, Fachhochschule Mannheim, D-68163 Mannheim 6 Max-Planck-Institut für Biochemie, D-82152 Martinsried Present address: Neurologische Klinik und Poliklinik Grosshadern, Klinikum der LMU München, D-81377 München
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Subcellular localization and in vivo subunit interactions of ubiquitous mu-calpain.
Ubiquitously expressed calpains are Ca(2+)-dependent, intracellular cysteine proteases comprising a large catalytic subunit (domains DI-DIV) and a noncovalently bound small regulatory subunit (domains DV and DVI). It is unclear whether Ca(2+)-induced calpain activation is followed by subunit dissociation or not. Here, we have applied advanced fluorescence microscopy techniques to study calpain ...
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